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One PUL encoding lyases from PL6, 7, and 17 families may be used for the degradation of alginate.

Strain S12 harbors a diverse array of CAZymes at multiple polysaccharide utilization loci (PUL). japonica fragments as the sole nutrient source within 48h. Results: Strain S12 degraded dehydrated L. We determined the enzymatic activity of strain S12 using the DNS method and measured its growth curve under different carbon sources using spectrophotometry. Methods: We sequenced the entire genome of strain S12 and constructed a phylogenetic tree using the core genome sequences of related strains. S12, a novel strain of marine flavobacteria that can degrade alginate and Laminaria japonica biomass, isolated from the intestines of the sea cucumber Apostichopus japonicas collected at Weihai coast. Carbohydrate-active enzymes (CAZymes) are the primary feature of marine bacteria that utilize these polysaccharides. Introduction: Macroalgae contain various polysaccharides that serve as nutrient sources Introduction: Macroalgae contain various polysaccharides that serve as nutrient sources for marine bacteria.